Potential role of stem cells from human exfoliated deciduous teeth in inducing liver regeneration

Abstract

AbstractBackground and AimEven with advancement of medical technologies, liver transplantation still faces several major challenges. Hence, other treatment modalities are urgently needed for patients with end‐stage liver disease. Stem cells from human exfoliated deciduous teeth (SHED) was discovered to have highly proliferative and pluripotent properties; including differentiation into hepatocyte‐like cells. This study aims to investigate the capability of intrasplenic transplanted SHED and SHED‐Hep cells in inducing proliferation of stem cells and native hepatocytes in order to accelerate liver regeneration in liver fibrosis mice models.MethodsThree carbon tetrachloride (CCl4)‐injured male mice groups were used in this study. Two of those groups were transplanted with either SHED or SHED‐Hep, while the other did not undergo transplantation. One age‐ and sex‐ matched healthy mice group was used as control. All specimens were immunohistochemically stained with anti‐Ki‐67 antibodies and anti‐proliferating cell nuclear antigen (PCNA) antibodies before counter stained with hematoxylin–eosin.ResultsAnti‐Ki‐67 antibodies staining: at both 8 and 12 weeks, proliferating activity was predominantly seen on both SHED‐ and SHED‐Hep‐transplanted CCl4‐injured mice groups, while control and non‐transplanted CCl4‐injured mice group showed little to no sign of proliferation activity. Anti‐PCNA staining: at both 8 and 12 weeks, significant proliferating activity was detected by PCNA staining, mainly on stem cells population area on SHED‐ and SHED‐Hep‐treated group.ConclusionsIn conclusion, this study has provided the evidence that transplantation of SHED or SHED‐Hep on liver‐injured mice induced proliferation of both transplanted stem cells and native liver cells in order to accelerate liver regeneration.