The extent of regeneration is impacted by the stage of amputation in ladybird beetles: A case study in Cheilomenes sexmaculata

Abstract

AbstractRegeneration has been a topic of interest across a range of taxa for centuries, and arthropods are no exception. Trade‐offs associated with regeneration are likely to involve the reallocation of resources away from other metabolic activities such as growth, development or reproduction. This might be reflected in costs to some developmental traits of the organism, despite regeneration being advantageous. These associated costs might also differ with the stage of injury or amputation. Here, we hypothesise that the extent of regeneration and trade‐offs associated with it may be stage‐specific. To test this hypothesis, the right forelimb of four larval stages of the ladybird beetle Cheilomenes sexmaculata (Fabricius) (Coleoptera: Coccinellidae) was amputated. Amputated individuals were reared until adulthood, and all developmental transitions were recorded. Regenerated legs in all the treatments were smaller than the controls, which did not experience the amputation, and the regenerative potency of early larval stages was higher than that of late larval stages. Limb regeneration caused delays in post‐amputation developmental duration in all the treatments, increasing their total developmental period. The length of the unamputated left foreleg as well as the wing and antenna size were also reduced in regenerated beetles, showing some internal trade‐off. However, there were no significant differences observed between regenerated and control adults in their fresh body weight and body size. Thus, limb regeneration depends upon the stage of larval development at which the amputation was performed. Amputation also affects the development of other appendages. The delay in normal beetle development might have been observed because of extra resource requirement, their allocation as well as reprogramming of the expression of some genes during regeneration.