Clinical evaluation of culturable bacteria, endotoxins and lipoteichoic acid in teeth with vital normal pulp tissues

Author:

Chiarelli‐Neto Vito M.1,de Aveiro Emelly1,Bronzato Juliana D.1ORCID,Arruda‐Vasconcelos Rodrigo1,Louzada Lidiane M.1,Godoi Ederaldo P.1,Lopes Erica M.1,de‐Jesus‐Soares Adriana1,Ferraz Caio C. R.1,Almeida José F. A.1,Marciano Marina A.1,Gomes Brenda P. F. A.1ORCID

Affiliation:

1. Division of Endodontics, Department of Restorative Dentistry Piracicaba Dental School – State University of Campinas – UNICAMP Piracicaba Brazil

Abstract

AbstractThe objective of this study was to investigate the presence of culturable bacteria, endotoxins (LPS) and lipoteichoic acid (LTA) levels in teeth with normal vital pulp (NVP) with intact crowns (IC) and those with coronal restoration (CR) limited to the enamel level. A total of 20 teeth indicated for endodontic treatment due to prosthetic reasons were selected. Samples were collected from the root canals. The levels of cultivable bacteria, LPS and LTA were assessed. Statistical analyses were performed at significance level set at 5%. None of the teeth presented microbial growth. In the IC group, the LPS levels were limited to the lowest concentration of LPS. On the contrary, higher LPS and LTA levels were detected in teeth with CR. It was concluded that teeth with NVP and IC were negative for bacteria, LPS and LTA; while teeth with CR were positive for bacterial virulence factors.

Funder

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Fundação de Amparo à Pesquisa do Estado de São Paulo

Fundo de Apoio ao Ensino, à Pesquisa e Extensão, Universidade Estadual de Campinas

Publisher

Wiley

Subject

General Dentistry

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