Proteomics analysis reveals the inactivation mechanisms of Alicyclobacillus acidoterrestris spores by atmospheric cold plasma

Author:

Wang Lang‐Hong1,Yan Bing1,Wen Qing‐Hui2,Wei Gui‐Feng1,Huang Yanyan1,Zeng Xin‐An13,Ali Murtaza1,Aadil Rana Muhammad4ORCID

Affiliation:

1. Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing, School of Food Science and Engineering Foshan University Foshan 528225 China

2. School of Health Jiangxi Normal University Nanchang 330022 China

3. School of Food Science and Engineering South China University of Technology Guangzhou 510641 China

4. National Institute of Food Science and Technology University of Agriculture Faisalabad 38000 Pakistan

Abstract

SummaryAlicyclobacillus acidoterrestris is a typical endospore‐forming microorganism, whose spores are able to germinate in Not From Concentrate juice causing its spoilage. In this work, dielectric barrier discharge atmospheric cold plasma (DBD‐ACP) at the voltage of 30 kV was used to inactivate Alicyclobacillus acidoterrestris spores in NFC juice (2–8 min). The results showed spores reduced nearly 3‐log CFU mL−1 after 8 min treatment, and the morphology of spores were severely disrupted, causing the release of their contents. Furthermore, proteomic analysis showed totals of 122 and 170 different expression proteins were detected in group C (control)/Ct1 (30 kV, 4 min) and C/Ct2 (30 kV, 8 min), respectively, indicating that metabolic activities of spores including amino acid and energy metabolic, protein biosynthesis, DNA replication and spore germination are inhibited. This study confirms the inactivation of Alicyclobacillus acidoterrestris spores by DBD‐ACP and provides some new perspectives on the inactivation mechanisms of spores by DBD‐ACP.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Guangdong Province

Publisher

Wiley

Subject

Industrial and Manufacturing Engineering,Food Science

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