In vitro hair growth‐promoting effect of Lgr5‐binding octapeptide in human primary hair cells

Abstract

AbstractBackgroundHair loss occurs due to various biological and environmental causes, which can have psychosocial consequences. The Wnt/β‐catenin signaling is well‐known for its role in hair growth and regeneration, as it induces the proliferation and differentiation of hair cells. When the leucine‐rich G protein‐coupled receptor 5 (Lgr5) interacts with the R‐spondins, the frizzled receptor (FZD), a Wnt receptor, becomes stabilized, resulting in an increased β‐catenin activity.AimWe investigated whether the octapeptide that binds to Lgr5 enhances proliferation and differentiation of human primary hair cells through the activation of Wnt/β‐catenin signaling.MethodsThe binding affinity of the octapeptide to Lgr5 was evaluated using surface plasmon resonance (SPR). We confirmed changes in proliferation and related factors like β‐catenin activation and growth factors (GFs) expression in human hair follicle dermal papilla cells (HHFDPCs). Additionally, we observed the proliferation and the expression of differentiation markers in human hair follicle outer root sheath cells (HHFORSCs), human hair follicle germinal matrix cells (HHFGMCs), and human hair follicle stem cells (HHFSCs). We used three‐dimensional HHFDPC spheroid culture treated with dihydrotestosterone (DHT) to create in vitro conditions that mimic androgenetic alopecia, and we studied the effects of octapeptide on Wnt expression and HHFSC differentiation.ResultsThe binding of the octapeptide to Lgr5 was confirmed using SPR analysis. In HHFDPCs, treatment with octapeptide resulted in a concentration‐dependent increase in proliferation. We also observed increased nuclear translocation of β‐catenin and increased expression of its downstream targets. HHFDPCs treated with octapeptide exhibited increased expression of growth factors and phosphorylation of Akt and ERK. In addition, we confirmed that octapeptide increased proliferation and induced differentiation in HHFORSCs, HHFGMCs, and HHFSCs. Under the HHFDPC spheroid culture conditions, we found that octapeptide restored the inhibition of Wnt‐5a and Wnt‐10b expressions by DHT. In HHFSCs treated with HHFDPC spheroid culture media, we observed that octapeptide recovered the inhibition of differentiation by DHT.ConclusionWe found that octapeptides activated the Wnt/β‐catenin signaling and induced the proliferation and differentiation of human primary hair cells by acting as an exogenous ligand for Lgr5. In addition, octapeptides recovered inhibited hair regeneration characters by DHT in androgenetic alopecia‐mimic in vitro model. These findings suggest that octapeptides may be a promising therapeutic option for treating hair loss.