Affiliation:
1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture Nanjing Agricultural University Nanjing China
2. Department of Plant Pathology University of Agriculture Faisalabad Pakistan
3. Department of Plant Pathology The Islamia University of Bahawalpur Bahawalpur Pakistan
4. College of Electrical and Mechanical Engineering, NUST Rawalpindi Pakistan
Abstract
Abstract
Aims
The potential of endophytic Bacillus strains to improve plant growth and yield was evaluated.
Methods and results
Endophytic Bacillus altitudinis HNH7 and Bacillus velezensis HNH9 were evaluated for their growth-promoting traits. In an in vitro plate assay, HNH7 and HNH9 exhibited proteolytic, amylolytic, lipolytic and cellulolytic activity. HNH7 and HNH9 were able to solubilize iron by producing siderophores but were unable to solubilize insoluble phosphate. PCR confirmed the presence of four growth-promoting genes viz. pvd, budA, asbA and satA in the genome of HNH7, while HNH9 also possessed the same genes except for budA. In a greenhouse experiment, HNH7 and HNH9 promoted the growth of upland cotton plants by upregulating the expression of growth-linked genes, EXP6, ARF1, ARF18, IAA9, CKX6 and GID1b. However, the expression of genes involved in ethylene biosynthesis, that is ERF and ERF17 was downregulated after treating the plants with HNH7 and HNH9 compared to the control. Furthermore, cotton plants treated with HNH7 and HNH9 exhibited a significantly higher rate of photosynthesis and stomatal conductance.
Conclusion
HNH7 and HNH9 showed a promising potential to promote the growth of cotton plants.
Significance and impact of study
Research on plant growth-promoting Bacillus strains can lead to the formation of biofertilizers.
Funder
National Natural Science Foundation of China
Postgraduate Research & Practice Innovation Program of Jiangsu Province
Publisher
Oxford University Press (OUP)
Subject
Applied Microbiology and Biotechnology,General Medicine,Biotechnology
Cited by
11 articles.
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