Integrated conjugal plasmid <scp>pLS20</scp> in the Bacillus subtilis genome produced 850‐kbp circular subgenomes transmissible to another B. subtilis-Reference-Cited by-同舟云学术

Integrated conjugal plasmid pLS20 in the Bacillus subtilis genome produced 850‐kbp circular subgenomes transmissible to another B. subtilis

Published:2024-04-25 Issue:7 Volume:29 Page:584-588
ISSN:1356-9597
Container-title:Genes to Cells
language:en
Short-container-title:Genes to Cells

Author:

Itaya Mitsuhiro¹^ORCID,Kataoka Masakazu¹

Affiliation:

1. Department of Biomedical Engineering Graduate School of Science and Technology Shinshu University Nagano Japan

Abstract

AbstractBacillus subtilis was engineered to produce circular subgenomes that are directly transmittable to another B. subtilis. The conjugational plasmid pLS20 integrated into the B. subtilis genome supported not only subgenome replication but also transmission to another B. subtilis species. The subgenome system developed in this study completes a streamlined platform from the synthesis to the transmission of giant DNA by B. subtilis.

Publisher

Wiley

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1111/gtc.13120

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