Abstract
Hyperglycemia is a condition in which the blood glucose levels exceed normal limits. Ethanol extract of Jamaican cherry leaves (Muntingia calabura L.) contains active components that have the potential to lower blood sugar levels and heal pancreatic cell damage in rats. Gut microbiota imbalance can cause immune system abnormalities, illnesses, and metabolic disorders, including diabetes. The aim of the present study was to determine whether an ethanol extract of Jamaican cherry leaves can repair pancreatic cell damage, as well as influence the total coliforms and lactic acid bacteria in hyperglycemic mice. In this study, 25 mice were randomly placed into five groups, which were then provided with food and drinking water. Alloxan in a dose of 160 mg/kg bw was administered to the positive control group and three treatment groups. Each of the three groups was then treated with the ethanol extract of Jamaican cherry leaves at doses of 300, 400, or 500 mg/kg bw for 14 days. The groups that received the extract were able to repair the pancreatic damage considerably by increasing the number of normal pancreatic cells. This was supported by histological observations. The total abundance of lactic acid bacteria in hyperglycemic mice was 1.08 ± 45 x 1010 CFU/g, which was substantially lower than that in mice treated with the extract. The overall coliform abundance in hyperglycemic mice was 4.37 ± 41 x 1010 CFU/g, which was higher than that in mice supplemented with the extract. Therefore, the administration of Jamaican cherry leaf ethanol extract on a regular basis can increase the abundance of cecum microbiota in hyperglycemic mice. Furthermore, this extract can be used as a natural alternative treatment for recovering pancreatic cell damage.
Publisher
Journal of Pure and Applied Microbiology
Subject
Applied Microbiology and Biotechnology,Microbiology,Biotechnology
Reference30 articles.
1. 1. Yang X, Zhang R, Jin T, et al. Stress Hyperglycemia Is Independently Associated with Persistent Organ Failure in Acute Pancreatitis. Dig Dis Sci. Published online 2021. doi: 10.1007/s10620-021-06982-8
2. 2. Giri B, Dey S, Das T, Sarkar M, Banerjee J, Dash SK. Chronic Hyperglycemia Mediated Physiological Alteration and Metabolic Distortion Leads to Organ Dysfunction, Infection, Cancer Progression and Other Pathophysiological Consequences: An update on glucose toxicity. Biomed Pharmacother. 2018;107:306-328. doi: 10.1016/j.biopha.2018.07.157
3. 3. Mouri Mi, Badireddy M. Hyperglycemia. StatPearls; 2022. https://www.ncbi.nlm.nih.gov/books/NBK430900/
4. 4. Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res. 2001;50(6):537-546.
5. 5. Chukwunonso Obi B, Chinwuba Okoye T, Okpashi VE, Nonye Igwe C, Olisah Alumanah E. Comparative Study of the Antioxidant Effects of Metformin, Glibenclamide, and Repaglinide in Alloxan-Induced Diabetic Rats. J Diabetes Res. 2016;2016:1635361. doi: 10.1155/2016/1635361