Abstract
The germicidal efficacy of various physico-chemical agents against culturable bacterial cells and bacterial spores is well studied and reviewed; except for the dormant Viable But Non Culturable state (VBNCs). VBNC is a special physiological state, where the bacterium is not culturable in culture media, but remains alive, infective and virulent. The aim of this study is to evaluate the germicidal efficacy of three physico-chemical agents against the VBNC forms of destructive phytopathogen Ralstonia pseudosolanacearum. Effect of thermotherapy Formalin and Sodium Hypochlorite on these forms was evaluated using standard plate count and reverse culturability tests. The disinfectants were found equally effective against the culturable cells and VBNC forms at recommended concentrations.
Publisher
Journal of Pure and Applied Microbiology
Subject
Applied Microbiology and Biotechnology,Microbiology,Biotechnology
Reference29 articles.
1. 1. Safni I, Cleenwerck I, De Vos P, Fegan M, Sly L, Kappler U. Polyphasic taxonomic revision of the Ralstonia solanacearum species complex: proposal to emend the descriptions of Ralstonia solanacearum and Ralstonia syzygii and reclassify current R. syzygii strains as Ralstonia syzygii subsp. syzygii subsp. nov., R. solanacearum phylotype IV strains as Ralstonia syzygii subsp. indonesiensis subsp. nov., banana blood disease bacterium strains as Ralstonia syzygii subsp. celebesensis subsp. nov. and R. solanacearum phylotype I and III strains as Ralstonia pseudosolanacearum sp. nov. Int J Syst Evol Microbiol. 2014;64(9):3087-3103. doi: 10.1099/ijs.0.066712-0
2. 2. Hayward AC, Hartman GL. Bacterial wilt: the disease and its causative agent, Pseudomonas solanacearum. In Bacterial wilt: the disease and its causative agent, Pseudomonas solanacearum. CAB International, Wallingford. 1994.
3. 3. Alvarez B, Biosca EG, Lopez MM. On the life of Ralstonia solanacearum, a destructive bacterial plant pathogen. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. 2010;1:267-279.
4. 4. Oliver JD. Recent findings on the viable but nonculturable state in pathogenic bacteria. FEMS Microbiol Rev. 2010;34(4):415-425. doi: 10.1111/j.1574-6976.2009.00200
5. 5. del Mar Lleo M, Benedetti D, Tafi MC, Signoretto C, Canepari P. Inhibition of the resuscitation from the viable but non‐culturable state in Enterococcus faecalis. Environ Microbiol. 2007;9(9):2313-2320. doi: 10.1111/j.1462-2920.2007.01345.x
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