Abstract
Keratinolytic microorganisms are highly valuable for decomposition of poultry waste. This study aimed to isolate keratin-decomposing actinobacteria from poultry farm soils and examine their capacity to decompose feathers. Soil samples were placed in a basal medium with feather meal, which is a deposit of carbon and nitrogen. Nine actinobacterial strains were isolated. Actinobacteria were cultured in the media to show clear feather-decomposing potential. Actinobacterial strains were identified using 16S ribosomal RNA (rRNA) sequencing as being related to Streptomyces rochei AM8. Thus, the supernatant of S. rochei AM8 exhibited keratinolytic enzyme activity. Increased biodecomposition of feathers was recorded in a keratinase assay (0.782 U/mL) for separated cultures. The ability of the selected microorganisms to decompose feathers may be an effective biotechnological solution for managing feather waste from poultry.
Publisher
Journal of Pure and Applied Microbiology
Subject
Applied Microbiology and Biotechnology,Microbiology,Biotechnology
Reference40 articles.
1. 1. Prasanna IL, Moorthy K. Isolation, Identification and Characterization of Feather Degrading Raoultella electrica from Poultry Waste Containing Soil. 2020.
2. 2. Khalel A, Alshehri W, Aly M. Enhancing Plant Growth by Chicken Feather Compost Obtained from Feather Degradation by Streptomyces enissocaesilis. Biosc Biotech Res Comm. 2020;13(4). doi: 10.21786/bbrc/13.4/32
3. 3. Manirujjaman M, Amin R. Nahid AA, Alam S. Isolation and characterization of feather degrading bacteria from poultry waste. African Journal of Bacteriology Research. 2016;8(3):14-21.
4. 4. Korkmaz H, Hur H, Dincer S. Characterization of alkaline keratinase of Bacillus licheniformis strain HK-1 from poultry waste. Ann Microbiol. 2004;54(2):201-211
5. 5. Ramnani P, Singh R, Gupta R. Keratinolytic potential of Bacillus licheniformis rg1: structural and biochemical mechanism of feather degradation. Can J Microbiol. 2005;51(3):191-196. doi: 10.1139/w04-123