Affiliation:
1. Voronezh State University of Engineering Technologies
2. Voronezh State University
Abstract
In order to ensure quality control and food safety, the in vivo effect of hops on the frequency of cell aberrations was studied for the first time using a micronucleus test in human buccal epithelium. Results were obtained of in vivo studies of wort samples to identify possible negative effects of hops, which contribute to an increase in extractive components in the finished drink during dry hopping and contain a large number of biologically active compounds (bitter and phenolic substances, essential oils), on the human body. The frequency of nucleus abnormalities in human buccal epithelium cells of persons using hoped and unhopped must samples was analyzed for the frequency of cellular abnormalities in human buccal epithelium. The following types of disorders were found: cells with micronuclei, two nuclei, notches, perinuclear vacuoles, "tongue" and "broken egg" type protrusions, karyopyknosis, karyolisis and karyorexis. Based on the data obtained, indices of repair and accumulation of cytogenetic disorders were calculated. No effect of hops on the reparation index and the index of accumulation of cytogenetic disorders was revealed. Factor analysis methods show that the main contribution to the variance of the trait system will be made by the factor "specificity of the cytogenetic indices under study".
Publisher
FSBEI HE Voronezh State University of Engineering Technologies
Subject
General Agricultural and Biological Sciences
Reference14 articles.
1. 1 Malowicki M. G., Shellhammer T. H. Isomerization and degradation kinetics of hop (Humulus lupulus) acids in a model wort-boiling system. Journal of agricultural and food chemistry. 2005. 53. no. 11. pp. 4434–4439. DOI:10.1021/jf0481296.
2. 2 Jaskula B., Goiris K., van Opstaele F., Rouck G. de, Aerts G., Cooman L. de. Hopping technology in relation to α-acids isomerization yield, final utilization, and stability of beer bitterness. Journal of the American Society of Brewing Chemists. 2018. 67. no. 1. pp. 44–57. DOI:10.1094/ASBCJ-2009-0106-01.
3. 3 Novikova I. V., Rukavitsyn P. V., Murav'ev A. S. Optimization of technological parameters of the dry-climbing process with the development of an integrated beer quality assessment methodology. Khranenie i pererabotka sel'khozsyr'ya [Storage and processing of agricultural raw materials]. 2021. pp. 163-175. DOI: 10.36107/spfp.2021.234 (in Russian).
4. 4 Novikova I. V., Rukavitsyn P. V., Murav'ev A. S. The influence of the main parameters of the dry hangover process on the physicochemical indicators of beer. Tekhnologiya i tovarovedenie innovatsionnykh pishchevykh produktov [Technology and product science of innovative food products]. 2018. pp. 9–17. (in Russian).
5. 5 Kalaev V. N., Artyukhov V. G., Nechaeva M. S. Micronuclear test of the beechkal epithelium of the human oral cavity: problems, achievements, prospects. Tsitologiya i genetika [Cytology and genetics]. 2014. pp. 62–80. (in Russian).