The viscoelasticity, anisotropy and location-dependence of mechanical properties of rabbit iris investigated using uniaxial tensile tests

Abstract

Purpose Abnormal iris mechanical properties have been considered to be an important cause of pupillary-block and angle-closure glaucoma. In this research, viscoelasticity, anisotropy and location-dependence of mechanical properties of rabbit iris were investigated using uniaxial tensile test. Methods Iris strips were taken along three directions: inner-circumferential direction (ICD), outer-circumferential direction (OCD) and radial direction (RD), respectively. Quasi-static tensile tests and stress-relaxation tests were applied on the iris strips. Then the stress-stretch data was fitted with third order Ogden model; the stress-relaxation data was fitted with the third order Prony series model. Through comparing the tangent modulus and relaxation limit of the strips from different directions and locations, the viscoelasticity, anisotropy and location-dependence of mechanical properties of rabbit iris were explored. Results The tangent moduli of iris at the stretch of 1.05 along ICD, OCD, and RD were 3.2 ± 1.4 kPa, 4.2 ± 2.6 kPa and 1.5 ± 0.8 kPa, respectively. Iris strips in ICD and OCD were found to have almost the same stress-relaxation behavior, and both relaxed slower than iris strips in RD. Conclusions The mechanical properties of the iris were nonlinear, viscoelastic, anisotropic and location-dependent. The stress growth rate of the circumferential direction iris strip is significantly lower than that of RD, and the stress relaxation rate is significantly higher than that of RD. That is, the iris is more prone to deformation in RD. This study can provide more precise iris material parameters for exploring the mechanical causes of pupillary-block.