Mitochondria-independent morphological and biochemical apoptotic alterations promoted by the anti-tumor agent bleomycin inSaccharomyces cerevisiae

Author:

Aouida Mustapha12,Mekid Halima12,Belhadj Omrane12,Mir Lluis M.12,Tounekti Omar12

Affiliation:

1. Laboratoire de Vectorologie et Transfert de Gènes, UMR 8121 CNRS, Institut Gustave–Roussy, 39 rue Camille Desmoulins, 94805 Villejuif CÉDEX, France.

2. Laboratoire de Biochimie et Technobiologie, Faculté des Sciences de Tunis, Campus universitaire, 1060 Tunis, Tunisie.

Abstract

Bleomycin is a highly potent cytotoxic and genotoxic agent used in the chemotherapy of various types of tumors. It is a radiomimetic anticancer drug that produces single- and double-stranded DNA breaks in a catalytic way. Using Saccharomyces cerevisiae as a model system, we show that when a high amount of bleomycin molecules is internalized (100 µmol/L), morphological changes identical to those usually associated with apoptosis, i.e., a sub-G1region peak, chromatin condensation, and very rapid DNA fragmentation into oligonucleosomal-sized fragments, are observed. The known bleomycin inhibitors cobalt and EDTA were able to prevent bleomycin nucleasic activity and thus apoptotic cell death. However, both oligomycin, a potent inhibitor of the mitochondial F0F1-ATPase, and antimycin, a drug affecting mitochondria respiration, were unable to prevent the bleomycin-induced apoptotic-like cell death. These results suggest that high bleomycin concentrations induce an apoptosis-like mitochondria-independent cell death in yeast.

Publisher

Canadian Science Publishing

Subject

Cell Biology,Molecular Biology,Biochemistry

Cited by 18 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3