Automated chromatographic analysis of free monosubstituted guanidines in physiological fluids

Author:

Durzan D. J.

Abstract

An automated analytical method for the quantitative determination of monosubstituted guanidines in the physiological fluids of plants and insects is described with special reference to conifers and spruce budworm larvae. Over 25 monosubstituted guanidines of known structure can be separated on columns of an amino acid analyzer of the Spackman, Stein, and Moore type, and determined by the Sakaguchi reagent as modified by Satake and Luck. The automated method minimizes the problem of color decay of the reaction product of the guanidine derivative with the Sakaguchi reagent and reveals the occurrence of several yet unidentified guanidines.

Publisher

Canadian Science Publishing

Subject

General Medicine

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