The accuracy center of a eukaryotic ribosome

Abstract

Mutations in yeast ribosomal proteins and ribosomal RNAs have been shown to affect translational fidelity. These mutations include: proteins homologous to Escherichia coli's S4, S5, and S12; a eukaryote specific ribosomal protein; yeast ribosomal rRNA alterations at positions corresponding to 517, 912, and 1054 in 16S E. coli rRNA and to 2658 in the sarcin–ricin domain of 23S E. coli rRNA. Overall there appears to be a remarkable conservation of the accuracy center throughout evolution.Key words: ribosomal RNA, ribosomal protein, yeast, translational accuracy.