Abstract
Glycine and formaldehyde are converted into serine in the presence of a dialyzed, lyophilized enzyme preparation from corn seedlings, with tetrahydropteroylglutamic acid and pyridoxal phosphate as coenzymes. The equilibrium constant of the reaction was calculated for the system at 37.5 °C as K = 3.1 × 103. The pH activity curve showed a maximum between 6.6 and 7.0. The effects of the concentrations of the coenzymes were studied. The glycine–serine interconverting enzyme is present throughout the seedling and occurs exclusively in the soluble fraction of the cells.
Publisher
Canadian Science Publishing
Cited by
10 articles.
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1. Ammonia Assimilation and Amino Acid Metabolism;Nucleic Acids and Proteins in Plants I;1982
2. Synthesis and Interconversion of Glycine and Serine;Amino Acids and Derivatives;1980
3. One-Carbon Metabolism**Abbreviations: The recommendations of the IUPAC-IUB Commission are followed in designation of pteroylglutamic acid and its derivatives, e.g., 10-HCOH4PteGlu, N10-formyltetrahydropteroylmonoglutamate, 5,10-CH2 H4 PteGlu, N5, N10-methylenetetrahydropteroylmonoglutamate, and 5-CH3H4PteGlu, N5-methyltetrahydropteroylmonoglutamate [Biochem. J. 102, 19–20 (1967)]. The term “folate” is used to denote metabolically active pteroylglutamates. Other abbreviations are AdoMet—S-adenosylmethionine, SAHC—S-adenosylhomocysteine γ-GCP—γ-glutamylcarboxypepidase, E.C. 3.4.12.10.;Metabolism and Respiration;1980
4. Folate derivatives of photosynthetic tissues;Phytochemistry;1972-11
5. Light quality in relation to growth, photosynthetic rates and carbon metabolism in two species of marine plankton algae;Marine Biology;1971-07