Transmembrane potential and force of contraction of the normal and potassium-deficient human atrial tissue in vitro

Abstract

The characteristics of the simultaneously recorded action potential (AP) and contraction of isolated atrial tissue of human heart were studied in normal and in potassium-free solutions. Two types of action potentials associated with characteristic contractions were observed. Pacemaker types of action potentials with two humps in the plateau were observed in spontaneously beating atria and they were associated with triple contractions. A non-pacemaker type of action potential was found in quiescent fibers when the preparation was driven electrically; this resulted in single peak contraction. The sizes of the resting and action potentials of pacemaker cells were lower, while those of action potential duration in all types of cells were higher than those reported in other mammals. When spontaneously beating atrial pieces were exposed to a potassium-free solution there was a shortening of the action potential plateau and lengthening of the terminal phase of repolarization associated with the development of the first hump into a slow spike. The shortening of the action potential plateau associated with an increase in the force of contraction was also observed in electrically stimulated muscles in KCl-free solution.