In vitro initiation of adventitious buds and its modification by high concentration of benzyladenine in leaf tissues of mulberry (Morus alba)

Abstract

Leaf explants of mulberry (Mortis alba L.) derived from aseptically grown shoots and seedlings were cultured on Murashige and Skoog's medium. Normal and abnormal leaves were grown by varying the concentration of benzyladenine. They differed in the way of forming adventitious buds. In normal leaves bud initiation occurred exclusively at the cut ends of midribs after removing petioles, while in abnormal ones buds formed at any region on midribs and petioles. Histological observations were made to study different patterns of bud initiation.