Author:
Forrester P. I.,Hancock R. L.
Abstract
L-Phenylalanine: tRNA ligase from E. coli was successfully isolated by affinity chromatography on a column of Sepharose-phenylalanine. The phenylalanine was separated from the Sepharose backbone by two molecules of ε-aminocaproic acid to ensure that there was a minimum of steric interference between the enzyme and the solid matrix.
Publisher
Canadian Science Publishing
Cited by
18 articles.
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