Author:
Duchesne Luc C.,Lelu Marie-Anne,Aderkas Patrick von,Charest Pierre J.
Abstract
A particle bombardment protocol using the Dupont Biolistic™ particle delivery system and the β-glucuronidase reporter gene was developed for embryogenic cell lines of Larix. Comparison of four sizes of tungsten microprojectiles showed the highest transient gene expression with 1.11 μm diameter particles. The highest β-glucuronidase transient gene activity was in cells bombarded 5 and 6 days after subculturing to fresh media. Comparison of 22 different cell lines of haploid and diploid Larixdecidua, Larixleptolepis, LarixXleptoeuropae, and LarixXeurolepis showed variation among the cell lines in β-glucuronidase gene activity, embryogenesis, and cell growth. In diploid lines, the levels of transient gene expression were higher with youngest lines; however, this trend was not distinguishable in haploid lines
Publisher
Canadian Science Publishing
Subject
Ecology,Forestry,Global and Planetary Change
Cited by
26 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献