Synthesis of soluble dextran–hemoglobin complexes of different molecular sizes

Abstract

Experimental conditions were defined that determined the synthesis of dextran–hemoglobin complexes through the alkylation of hemoglobin by N-bromoacetylaminoethylaminodextran. Using appropriate concentrations of the two reactants, over 90% yield of dextran–hemoglobin was obtained for dextrans of average molecular weight of 200 000, 110 000, 70 000, 40 000, and 20 000. Extensive viscosity increase due to crosslinking could be avoided, and a large molar excess of dextran over hemoglobin made unnecessary, under the optimal conditions.