Sporulation de Entomophthora obscura Hall & Dunn en culture liquide

Abstract

Growth and production of azygospores of Entomophthora obscura were studied through batch cultures in media containing glucose, or vegetable oil, and yeast extract. In media containing 3% glucose and 1% yeast extract, an 8-h lag phase occurred and sporulation began only at the 40th hour of culture; spore maturation lasted 4 days in average. Young mycelial stages were characterized by high nucleic acid and protein but low lipid concentrations. During sporogenesis stages, the quantity of lipids and chitin increased and the concentration of total polyosides (chitin excluded) was lower in comparison with the mycelial stages. Sporulation was induced by means of medium starvation in carbon and (or) nitrogen. The only sporulation inhibitor which allowed considerable growth was sodium fluoroacetate. [Translated by the journal]