Studies on cell fusion and the rescue of SV40 from cell cultures of successive generations of Cl2TSV5- and RHaT-produced hamster tumors

Abstract

Five successive generations of tumors have been produced in inbred Syrian hamsters after initial inoculation of SV40-transformed Cl2TSV5 and RHaT cell lines.SV40 could be rescued by cell fusion with inactivated Sendai virus from cell cultures of all five tumor generations. T-antigen was detected in all cultures by fluorescent antibody staining.An increased capacity for fusion of SV40-transformed cells after successive passages in hamsters has been observed.In addition to monkey kidney cells, bovine and lapine kidney cells have been successfully used for SV40 rescue by cell fusion.