THE RESOLUTION OF URINARY OR SERUM PROTEINS BY CHROMATOGRAPHY ON DEAE CELLULOSE COLUMNS WITH PARTICULAR REFERENCE TO URINARY PROTEINS AFTER THERMAL BURNS

Abstract

Serum proteins were separated into the usual electrophoretic fractions by the starch block technique. Each fraction was then examined by DEAE cellulose chromatography and by starch gel electrophoresis. The combination of starch block electrophoresis and DEAE cellulose chromatography was able to distinguish 22 different serum proteins.The application of DEAE cellulose to the chromatographic resolution of urinary proteins is described. The urine was filtered and the protein was concentrated by ultrafiltration. After dialysis, a suitable aliquot was adsorbed on a DEAE cellulose column and eluted by buffers with a progressively increasing hydrogen ion concentration and ionic strength. Some illustrative chromatographs are given.A number of urines from patients with severe burns were examined by this procedure. The increase in protein excretion was found in all sections of the chromatographs with particular increases in the γ globulin and acid mucoprotein zones.