The direct measurement of ribosomal RNA gene activity in wheat–rye hybrids

Abstract

Cloned rDNA segments, from wheat (Triticum aestivum) and rye (Secale cereale), which included the entire spacer region plus parts of the flanking 18S and 26S rRNA genes, were used to assay transcripts formed in the nuclei from wheat–rye hybrids. The tetraploid triticales (× Triticosecale Wittmack) carrying a complete rye chromosome complement and a combination of wheat chromosomes, as well as wheat and rye per se, were studied. Nuclei isolated from young leaves were incubated in the presence of [32P]UTP for 10 min, after which the total RNA was isolated. Hybridization of this radioactive RNA to the cloned rDNA segments showed that the suppression of rye NOR activity, measured cytologically, can be directly related to a major reduction in rRNA synthesis.Key words: NOR suppression, RNA transcripts, isolated nuclei.