Abstract
The degradation of DNA in bacterial cells has been studied in two strains of Escherichia coli after exposure to X-radiation, ultraviolet (uv.), or methyl methanesulfonate. The degradative system functions differently in freshly harvested growing cells and in cells which have been aged by standing for 2 days at room temperature in saline-phosphate. Growing cells degrade their DNA most effectively at low doses of X-radiation and require a factor whose synthesis is inhibited by chloramphenicol; these cells also degrade DNA after exposure to uv. radiation. Aged cells degrade their DNA most effectively at high X-radiation doses, do not require the chloramphenicol-sensitive factor, and no longer respond to uv. irradiation. Together with data on effects of freezing, of cold-water, and of various inhibitors, the above results suggest that the system responsible for radiation-induced degradation of DNA is dependent on structural organization in the intact cell.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology
Cited by
2 articles.
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