Author:
Diena B. B.,Wallace R.,Greenberg L.
Abstract
A simple method for differentiation of bacteria parallel to Gram staining is described, using vital staining with blue tetrazolium, decolorizing the preparation with a 1:1 mixture of acetone – ethylene tetrachloride for 4 hours, and counterstaining it with carbol fuchsin. Gram-negative bacteria are decolorized while the Gram-positive ones (except B. cereus) retain their formazan. Similar results were obtained by decolorizing vitally stained bacteria with organic solvents by chromatography.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology
Cited by
2 articles.
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