Structural characterization of the capsular polysaccharide and O-chain of the lipopolysaccharide of Actinobacilluspleuropneumoniae serotype 8 (strain 405)

Abstract

The capsular polysaccharide of Actinobacilluspleuropneumoniae serotype 8 (strain 405) was found to be a teichoic acid type polysaccharide composed of 2-acetamido-2-deoxy-D-galactose (two parts), glycerol (one part), and phosphate (one part). From hydrolysis, dephosphorylation, methylation, and 1H and 13C nuclear magnetic resonance studies, the polysaccharide was found to be a high molecular weight linear polymer having the structure:[Formula: see text]The lipopolysaccharide O-antigen of A. pleuropneumoniae serotype 8 was shown to be a high molecular weight unbranched linear polymer of a repeating pentasaccharide unit having the structure identical to that produced by A. pleuropneumoniae serotype 3, as follows:[Formula: see text]Keywords: Actinobacilluspleuropneumoniae polysaccharide, lipopolysaccharide, NMR.