Author:
Evans Kirby J.,Filion W. Gary
Abstract
The BrdU-FPG method for differential staining of sister chromatids was employed to estimate the cell cycle time in two plants species. The protocol is based upon the ability to differentiate cytologically between chromosomes which have completed two or three DNA synthesis phases. Vicia faba L. which has a well established cell cycle time was used to verify the technique. Utilizing this method the cell cycle time of Zebrina pendula Schnizl. root tip meristem cells was determined to be 17.0 hours.
Publisher
Canadian Science Publishing
Subject
Cell Biology,Plant Science,Genetics
Cited by
6 articles.
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