Author:
Campbell Robert A.,Durzan Donald J.
Abstract
Hypocotyl segments of white spruce seedlings, when placed with their basal end in a defined medium containing agar and 6-benzylaminopurine (BAP), formed up to 100 or more scale-like organs per segment. The organs did not arise from a preexisting apical meristem. The induction was inhibited by α-naphthaleneacetic acid (NAA). On medium containing 10−5 M BAP and no NAA, 69% of the explants formed multiple organs. When explants bearing the scale-like organs were transferred to media containing neither BAP nor NAA, the organs grew out into needles, and buds developed. Many of the buds developed into elongated shoots.
Publisher
Canadian Science Publishing
Cited by
123 articles.
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