Enzymes of the intermediary carbohydrate metabolism of Polyangium cellulosum

Abstract

Crude extracts of vegetative cells of the cellulolytic myxobacter Polyangium cellulosum contained significant levels of the enzymes of the tricarboxylic acid cycle and the glyoxylate cycle. Key enzymes of glycolysis and the pentose phosphate shunt were also detected. Specific activities of hexokinase and fructose- 1,6-diphosphate aldolase exhibited a 10-fold increase when the cells were grown in complex medium containing glucose. Cytochromes of a, b, and c type were demonstrated. By the use of a dispersly growing strain of P. cellulosum, its generation time was determined to be 22–24 h. This study suggests that the organism probably uses glycolysis and citric acid cycle for complete oxidation of glucose. The exact role of the glyoxylate cycle and pentose phosphate shunt cannot be deduced from this study. This is the first report on the study of intermediary carbohydrate metabolism in any member of the family Polyangiaceae.