Structural characteristics of root–fungal interactions for five ericaceous species in eastern Canada

Abstract

A combination of light microscopy (including differential interference contrast) and laser scanning confocal microscopy was used to document the colonization patterns of epidermal cells and details of intracellular hyphal complexes of five native ericaceous hosts: Vaccinium oxycoccos L. (bog cranberry), Ledum groenlandicum Oeder. (Labrador tea), Vaccinium myrtilloides L. (velvet-leaf blueberry), Kalmia angustifolia L. (sheep laurel), and Gaultheria procumbens L. (wintergreen). Colonization patterns, hyphal complex morphology, and the structure of thick-walled epidermal cells varied considerably among hosts. Multiple hyphal connections were observed between adjacent epidermal cells, indicating that one fungal entry point may result in the colonization of more than one epidermal cell. Further field observations combined with fungal isolations from field-collected plants, identification, and reinoculation studies of other species in the large Ericaceae family are required to determine the full range of structural details in ericoid mycorrhizas.Key words: ericoid mycorrhizas, hair roots, intracellular hyphal complexes, confocal microscopy.