Sclerotia as viable sources of mycelia for the establishment of ectomycorrhizae

Abstract

The ectomycorrhizal fungi Pisolithus tinctorius and Paxillus involutus, which have wide host potential and diverse ecological ranges, were grown in association with pine seedlings in plastic growth pouches. Sclerotia formed under these conditions were stored at room temperature for up to 30 days and then germinated on agar medium. Pure cultures of P. involutus also produced sclerotia and these also regenerated in culture. Mycelial plugs from the sclerotia-derived cultures were subsequently reinoculated onto pine roots. Typical ectomycorrhizae with a mantle and Hartig net were established.