Comparison of two culture methods for the production of haploids by anther culture in Solanum chacoense

Abstract

The response of anther culture to two culture procedures was tested using anthers of a clone of self-incompatible Solanum chacoense Bitt. grown under field conditions and in a greenhouse. The use of a high level of sucrose (20%) in the induction medium increased the proportion of anthers producing macroscopic calli. This effect was more pronounced for plants grown under field conditions than for those grown in a greenhouse. The later regeneration process, using plants from either source, was not affected by this procedure.