Genetic analysis of Aspergillus nidulans unstable transformants obtained by the biolistic process
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Published:1998-12-01
Issue:12
Volume:44
Page:1137-1141
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ISSN:0008-4166
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Container-title:Canadian Journal of Microbiology
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language:en
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Short-container-title:Can. J. Microbiol.
Author:
Barcellos Fernando Gomes,Fungaro Maria Helena Pelegrinelli,Furlaneto Márcia Cristina,Lejeune Bernard,Pizzirani-Kleiner Aline A,Lúcio de Azevedo João
Abstract
Mitotically unstable Aspergillus nidulans argB+ transformants obtained by the biolistic process were studied in the present work. Hybridization signals from undigested DNA and pulsed-field chromosomal bands of the transformants suggested the introduced plasmid occurred as free concatenated molecules. Fifteen vigorous growth sectors released from the transformants were analysed in order to understand the mechanisms involved in their formation. All sectors showed the integration of exogenous genes into the fungal genome by homologous or heterologous recombinant events.Key words: Aspergillus nidulans, biolistic process, mitotic instability, fungal transformation.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology
Cited by
16 articles.
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