Author:
Froese A.,Sehon A. H.,Eigen M.
Abstract
The kinetics of protein–dye and antibody–hapten reactions were studied with the temperature-jump method. The systems used consisted of (i) bovine serum albumin (BSA) and the dye 1-naphthol-4-[4-(4′-azobenzene azo)phenyl arsonic acid], referred to as N—R′, (ii) BSA and the dye 1-naphthol-2-sulphonic acid-4-[4-(4′-azobenzene azo)phenyl arsonic acid], referred to as NS—R′, and (iii) rabbit antibodies to phenyl arsonic acid [Ab] and the hapten N—R′.Each of the systems exhibited a single relaxation time. From the analysis of the concentration dependence of the relaxation times, it was concluded that each system could be represented by the reactions[Formula: see text]where P refers to BSA or Ab, and D to N—R′ or NS—R′. The following rate constants were calculated for the three systems at 25 °C:[Formula: see text]The effects of temperature and pH on the rate constants of the system BSA – N—R′ are discussed.
Publisher
Canadian Science Publishing
Subject
Organic Chemistry,General Chemistry,Catalysis
Cited by
93 articles.
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