Author:
Martínez-Lage A,Rodríguez-Fariña F,González-Tizón A,Méndez J
Abstract
A phylogenetic reconstruction based on the amplification of 3 satellite DNAs (stDNAs) was carried out in 1 crustacean species and 15 bivalve species of the subclass Pteriomorphia (10, subfamily Mytilinae; 1, subfamily Litophaginae; 1, subfamily Modiolinae, all belonging to family Mytilidae; 1, family Arcidae; and 2, family Pectinidae). The sequences obtained showed motifs with high similarity to those of A and B boxes of tRNA promoter regions. Dot-blot hybridizations revealed that the 3 stDNAs are present mainly in high copy numbers for each species of the genus Mytilus, whereas for the other species they appear in low copy numbers. Maximum-parsimony trees evidenced a tendency to group Mytilus clones together, and species containing these sequences as a single copy were distributed among the different mytilids. Finally, the possible origin and evolution of these stDNAs is discussed.Key words: bivalves, Pteriomorphia, satellite DNA, phylogeny, dot-blot.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,General Medicine,Biotechnology
Cited by
25 articles.
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