Environmental heterogeneity affects seasonal variation in thyroid hormone physiology of free-living arctic ground squirrels (Urocitellus parryii)

Abstract

Thyroid hormones (TH) are key regulators of metabolism that could play an important role in altering physiology and energy allocation across life-history stages. Here, we examine seasonal TH dynamics from 345 plasma samples collected from 134 free-living arctic ground squirrels (Urocitellus parryii (Richardson, 1825)) across three consecutive years (2014–2016). We also examine whether unbound levels of triiodothyronine (free T3) in plasma are correlated with total T3 levels and total thyroxine (T4) levels, and whether fecal T3 metabolite levels correlate with plasma TH levels. We found significant differences in plasma TH levels across stages of the annual cycle, but these differences were highly inconsistent across years. However, within a given time period, pregnant females had lower free T3 levels compared with lactating females. We also found that although free T3 was correlated with both total T3 and total T4 in plasma, fecal T3 was not positively correlated with plasma TH levels. Given the lack of consistent differences across life-history stages, we suggest environmental heterogeneity may be the primary driver of TH dynamics, masking any potential endogenous seasonal rhythms in TH secretion. We urge caution in the use of fecal T3 metabolites as a proxy for circulating levels and encourage further research to understand the observed discrepancy.