Endosporium, a new endoconidial genus allied to the Myriangiales

Abstract

A new endoconidial genus, Endosporium gen. nov., is reported from Alberta, Canada. Two species are described: Endosporium populi-tremuloidis Tsuneda sp. nov. (type species) isolated from a heavily colonized bud of Populus tremuloides Michx., and Endosporium aviarium Tsuneda sp. nov. isolated from the skin of Bombycilla garrulus (L.) (Bohemian waxwing) and a twig of Populus balsamifera L. This genus is morphologically similar to Phaeotheca in forming black, slow-growing colonies (conidiomata) containing numerous, conidiogenous, cellular clumps, but different in that (i) colonies possess aerial, determinate hyphae on the surface; (ii) mature colonies eventually collapse to release a mixture of conidiogenous cellular clumps, endoconidia, and blastic conidia in slimy liquid; and (iii) endoconidia are hyaline. Phylogenetic analyses of nuclear rDNA SSU, LSU, and ITS sequences indicate that Endosporium is phylogenetically distant from other endoconidial taxa, including Phaeotheca, and is most closely related to the Myriangiales (Dothideomycetes). Similarities in stroma and conidioma development as well as ecology between the two taxa suggest that Endosporium can be accommodated within this order, making it the first endoconidial and first black meristematic genus reported in the Myriangiales.