Soybean mitochondrial transcripts capped in vitro with guanylyltransferase

Abstract

A method was devised which permitted the efficient labelling of soybean mitochondrial transcripts carrying initiator 5′ di- or tri-phosphate termini with [α-32P]GTP and the capping enzyme guanylyltransferase. The RNAs labelled in this manner appear to represent several different transcription initiation sites and to be present at only low to moderate abundance in soybean mtRNA preparations.Key words: transcription initiation, promoter, mitochondrial DNA, RNA processing, gene expression.