The effect of 2,4-D and kinetin on the morphology, growth, and cytochemistry of peroxidase of cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

Abstract

Cotyledon cell suspension cultures of Phaseolus vulgaris were grown in the presence and absence of the growth regulators (2,4-dichlorophenoxy)acetic acid (2,4-D) and kinetin. Omission of the regulators changed the growth and cell division and produced striking changes in morphological characteristics. Cytochemical studies of peroxidase activity showed that the enzymes were mainly cytoplasmic in young cells and mainly associated with wall in older cells. No apparent differences in localization of activity were detected between the treatments, but cells cultured without the regulators showed a much more intense enzyme reaction. The location and increase of peroxidase, with increasing cell age and cell complexity, are consistent with the view that peroxidase activity is involved in cell wall expansion and differentiation.