Mammalian DNMTs in the male germ line DNA ofDrosophila-Reference-Cited by-同舟云学术

Mammalian DNMTs in the male germ line DNA ofDrosophila

Published:2008-10 Issue:5 Volume:86 Page:380-385
ISSN:0829-8211
Container-title:Biochemistry and Cell Biology
language:en
Short-container-title:Biochem. Cell Biol.

Author:

Weyrich Alexandra¹²³⁴⁵,Tang Xiaojing¹²³⁴⁵,Xu Guoliang¹²³⁴⁵,Schrattenholz André¹²³⁴⁵,Hunzinger Christian¹²³⁴⁵,Hennig Wolfgang¹²³⁴⁵

Affiliation:

1. DAAD-Laboratory, MPG-Guest Laboratory, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.

2. CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.

3. Institut für Genetik, Johannes Gutenberg-Universität, Mainz, Germany.

4. Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China.

5. ProteoSys, Carl Zeiss Str. 1, Mainz 55149, Germany.

Abstract

It is controversial whether DNA methylation plays a functional role in Drosophila . We have studied testis DNA of Drosophila melanogaster Meigen, 1830 with antisera against 5-methylcytosine (5mC) and found no evidence for the presence of significant amounts of 5mC. Reactions occur only with 1 of 3 5mC antisera, but they are restricted to nuclear regions without detectable amounts of DNA. The antisera apparently cross-react with other nuclear components. If the murine de novo DNA methyltransferases, DNMT3A and DNMT3B, are expressed under the control of the spermatocyte-specific β2-tubulin promoter in testes, DNA methylation is not increased and no effects on the fertility of the fly are seen. DNA methylation has, therefore, no functional relevance in the male germ line of Drosophila.

Publisher

Canadian Science Publishing

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

http://www.nrcresearchpress.com/doi/pdf/10.1139/O08-096

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