Author:
Bergh A. K.,Webb S. J.,McArthur C. S.
Abstract
In this study, no correlation was found between the Gram staining reaction of bacterial cells and the kinds of fatty acids in the lipids. The polar solvent (usually alcohol) used to wash out the stain from Gram-negative organisms removes much more lipid from these than from Gram-positive forms. The extractability of lipids from cells of Staphylococcus epidermidis increased when they became Gram-negative through aging. The infrared spectrum of the lipids extracted from these Gram-negative Staphylococci remained the same as that of the Gram-positive cells. It was further noted that high polarity characteristics and mutual miscibility in aqueous and lipid solvents are requirements of the decolorizing solvent.The Gram staining reaction of a cell appears to be related to the manner in which some of its lipids are bound to other components of the cell and the ease or difficulty with which the lipids are separated from lipoprotein and other complexes by certain polar solvents.
Publisher
Canadian Science Publishing
Cited by
10 articles.
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