HISTAMINE RELEASE BY HOMOLOGOUS PLASMA IN THE DOG

Abstract

Dogs were injected intracutaneously with their own plasma and with plasma from other dogs. The injected skin in each case was excised and incubated in Tyrode's medium. More free histamine was found in the media containing skin injected with homologous plasma than in the media containing skin injected with autologous plasma. When considered with earlier observations, this result justifies the conclusion that, in the dog, homologous plasma releases histamine on initial injection. Similar experiments with the histamine liberator compound 48/80 showed the method to be extremely sensitive in detecting histamine release.