Affiliation:
1. Key Laboratory of Genetics Breeding and Reproduction of Grass Feeding Livestock, Ministry of Agriculture and Rural Affairs, Urumqi 830000, China
2. College of Animal Science, Tarim University, Alar 843300, China
Abstract
The purpose of this study was to explore the potential expression regularity of POSTN in ovarian tissue. In this study, the uterus, ovarian follicles, heart, liver, spleen, lung, kidney, and muscle tissues of Merino sheep (10) were collected and detected by Western blot, real-time quantitative polymerase chain reaction, immunohistofluorescence, and fluorescence staining, respectively. The expression of POSTN in various organizations was analyzed. The results showed that the POSTN in Merino sheep had close homology with Bos mutus and Bos taurus. The expression of POSTN was detected in the uterus, follicle, heart, liver, spleen, lung, kidney, and muscle tissue, among which the expression of POSTN was highest in ovarian tissue. In addition, the expression of POSTN gradually increased with the increase of follicle diameter, among which POSTN was highly expressed in the granulosa cells (GCs) of follicles. Meanwhile, POSTN were distributed throughout the nucleus and cytoplasm of GCs, suggesting that POSTN may be involved in the regulation of follicle development.
Publisher
Canadian Science Publishing
Subject
Animal Science and Zoology,Food Animals