Comparative analysis of antimicrobial resistance and genetic characteristics of Escherichia coli from broiler breeder farms in Korea

Abstract

Broiler breeder farms could be a reservoir of Escherichia coli, disseminating antimicrobial resistance and virulence factors. We investigated the antimicrobial resistance of E. coli from nine broiler breeder farms and characterised their resistance and virulence genes. A total of 256 E. coli showed a high level of resistance to tetracycline, nalidixic acid, ampicillin, and cephalothin, followed by trimethoprim–sulfamethoxazole and chloramphenicol. The resistance to nalidixic acid, ampicillin, trimethoprim–sulfamethoxazole, and chloramphenicol showed significant differences among the farms. Among 202 β-lactam-resistant E. coli, 138 carried β-lactamase genes. The most prevalent β-lactamase gene was blaTEM-1, of which the presence differed significantly across the farms. Out of 197 tetracycline-resistant E. coli isolates, tetA and tetB were detected in 164 and 50, with significant differences among the farms. Also, 45 of 196 nalidixic acid-resistant E. coli carried qnrS, whereas 67 of 149 trimethoprim–sulfamethoxazole-resistant E. coli carried sul2. Among the five virulence genes tested, ompT was the most prevalent, and all genes except for iutA distributed significantly different among the farms. The phenotypic and genotypic characteristics of E. coli were significantly different among the farms; therefore, management at the breeder level is required to control the vertical transmission of E. coli.