Production et propriétés du complexe cellulasique du Botrytis cinerea

Abstract

The production of cellulasic complex by the fungal pathogen Botrytis cinerea is induced by addition of cellulose in the culture medium. The amount of activity increases greatly when the culture medium is supplemented with pectins, whereas glucose and cellobiose strongly inhibit the production of the complex. The cellulasic complex is constituted by three enzymes, separated and identified as β-glucosidase, exo 1,4-β-D-glucanase and endo 1,4-β-D-glucanase. The catalytic activity of the whole cellulasic complex is low and the inhibitors glucose and cellobiose act differently on this activity. Keywords: fungal pathogen, Botrytis cinerea, cellulasic complex, β-glucosidase, 1,4-β-D-glucanases.