Contractile function in vitro of slow-twitch skeletal muscle from weanling mice subjected to wasting malnutrition

Abstract

Our hypothesis was that malnutrition sufficient to produce weight loss in weanling mice would decrease the ability of slow-twitch skeletal muscle to develop and maintain force. We isolated muscles from 3 groups (n = 5) of weanling C57BL/6J mice of both sexes (i) mice at 19 days of age serving as zero-time or baseline controls (CONT) (ii) mice fed for the next 14 days with a low-protein diet that produces features of incipient kwashiorkor (LPD) and (iii) mice fed for the next 14 days with a complete diet (NORM). Muscles were also obtained from 5 adult mice 7–9 months of age (MAT). We stimulated the soleus at 50 Hz for 500 ms at 0.6 tetanic contractions per min (tet·min–1), 6 tet·min–1, and 30 tet·min–1in Krebs-Henseleit bicarbonate buffer at 27°C gassed with 95% O2and 5% CO2. The initial developed force (mN·mm–2) at 0.6 tet·min–1did not differ across groups (CONT 211.7 ± 16.0, LPD 274.2 ± 41.6, NORM 246.8 ± 38.0, MAT 210.8 ± 10.6). The fatigue rate (mN·mm–2·min–1) at 6 tet·min–1was significantly slower in muscles from CONT (0.6 ± 0.3) and LPD (0.6 ± 0.4) than in NORM (2.4 ± 0.6) and MAT (2.3 ± 0.2). At 30 tet·min–1, the fatigue rate (mN·mm–2·min–1) did not differ across groups (CONT 2.4 ± 0.5, LPD 2.7 ± 0.5, NORM 2.5 ± 0.4, MAT 2.0 ± 0.2). After stimulation at 6 tet· min–1and 30 tet·min–1, only muscles from CONT and LPD recovered to 100%. Because muscles from LPD mice developed equal force, fatigued less, and recovered from fatigue to a greater extent than muscles from NORM mice, we rejected the hypothesis. The function of the tissue remaining in the muscles from LPD mice approximated that of muscles from mice at 19 days of age rather than muscles from either mice of the same age fed a complete diet or adult mice.Key words: developed force, fatigue, recovery from fatigue.