Author:
Jakeman David L,Withers Stephen G
Abstract
Oligosaccharide synthesis by enzymatic processes offers the potential for thrusting oligosaccharides to the forefront of pharmaceutical research, in part, due to expedient and scalable reaction protocols. Glycosynthases are an emerging class of mutant enzymes capable of synthesizing glycosidic linkages in high yield. We report a new glycosynthase enzyme generated by a point mutation of E. coli β-galactosidase that condenses α-galactosyl fluoride with aryl glucosides forming a β-(1,6) glycosidic linkage. A further point mutation within the enzyme, proximal to the active site, increases the yields significantly.Key words: glycosyl transfer, glycosynthase, enzymatic oligosaccharide synthesis. tétrahydrooxazine, isofagomine, iminosucres, inhibiteurs de glycosidases.[Traduit par la Rédaction]
Publisher
Canadian Science Publishing
Subject
Organic Chemistry,General Chemistry,Catalysis
Cited by
24 articles.
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