The effect of hydroxyurea on DNA synthesis of Tipula iridescent virus and Estigmene acrea cells

Abstract

Viral-specific DNA synthesis of Tipula iridescent virus (TIV) was not affected in Estigmene acrea cells which were continuously exposed to 300 μg/mL hydroxyurea (HU) as detected by light microscope autoradiography. Electron microscopy of such cells showed viroplasmic centres with virions in various stages of development. In nor mal cells similarly exposed to HU, nuclear DNA synthesis was reduced by 70–80%.